mouse NDUFS1 shRNA silencing AAV

Name
AAV-m-NDUFS1-shRNA

Cat No
shAAV-265598

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Mouse Ndufs1.

shAAV-265598

AAV-m-NDUFS1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Ndufs1 (NADH dehydrogenase (ubiquinone) Fe-S protein 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

5830412M15Rik; 9930026A05Rik

Description (Vector)

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized.

Gene ID

227197

Gene Name (Vector)

NADH dehydrogenase (ubiquinone) Fe-S protein 1

Gene Symbol

Ndufs1

ORF Size (aa)

2184

ORF Size (bp)

2184 bp

RefSeq ID

NM_145518

RefSeq Synonyms

NM_145518, NM_001160040, NM_001160039, NM_001160038, BC015300, BC006660,

Species

mouse

UniGene ID

Mm.392955

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.