mouse MBD4 shRNA silencing AAV

Name
AAV-m-MBD4-shRNA

Cat No
shAAV-264315

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Mouse Mbd4.

shAAV-264315

AAV-m-MBD4-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Mbd4 (methyl-CpG binding domain protein 4). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

Med1

Description (Vector)

Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.

Gene ID

17193

Gene Name (Vector)

methyl-CpG binding domain protein 4

Gene Symbol

Mbd4

ORF Size (aa)

1665

ORF Size (bp)

1665 bp

RefSeq ID

NM_010774

RefSeq Synonyms

NM_010774, BC024812,

Species

mouse

UniGene ID

Mm.259308

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.