mouse LIN28B shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Lin28b.
shAAV-263462
AAV-m-LIN28B-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Lin28b (lin-28 homolog B (C. elegans)). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
2810403D23Rik; D030047M17Rik; Lin-28.2
Description (Vector)
Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.
Gene ID
380669
Gene Name (Vector)
lin-28 homolog B (C. elegans)
Gene Symbol
Lin28b
ORF Size (aa)
816
ORF Size (bp)
816 bp
RefSeq ID
NM_001031772
RefSeq Synonyms
NM_001031772, BC089037,
Species
mouse
UniGene ID
Mm.440328
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.