human GNE Over-expression AAV

Name 
AAV-h-GNE
Cat No 
AAV-210109
Availability
4-5 weeks
Categories
AAV, Over-Expression

This is an AAV expressing Human GNE.

AAV-210109
AAV-h-GNE

Ready-to-use AAV expressing Human GNE (glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
CMV (default), or choose from 30 different ubiquitous or cell specific promoter
Reporter
none, optional GFP, CFP, YFP, RFP or mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
DMRV; GLCNE; IBM2; NM; Uae1
Description (Vector)
GNE is a bifunctional enzyme that initiates and regulates the biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. It is a rate-limiting enzyme in the sialic acid biosynthetic pathway. Sialic acid modification of cell surface molecules is crucial for their function in many biologic processes, including cell adhesion and signal transduction. Differential sialylation of cell surface molecules is also implicated in the tumorigenicity and metastatic behavior of malignant cells. Mutations in this gene are associated with sialuria, autosomal recessive inclusion body myopathy, and Nonaka myopathy. Alternative splicing of this gene results in transcript variants encoding different isoforms.
Gene ID
10020
Gene Name (Vector)
glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
Gene Symbol
GNE
HGNC ID
HGNC:23657
ORF Size (aa)
2169
ORF Size (bp)
2169 bp
RefSeq ID
NM_005476
RefSeq Synonyms
NM_005476, NM_001190388, NM_001190384, NM_001190383, NM_001128227, BC121179,
Species
human
UniGene ID
Hs.5920

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.