mouse ITK shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Itk.
shAAV-262471
AAV-m-ITK-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Itk (IL2 inducible T cell kinase). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
Emt; Tcsk; Tsk
Description (Vector)
Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells/LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation.
Gene ID
16428
Gene Name (Vector)
IL2 inducible T cell kinase
Gene Symbol
Itk
ORF Size (aa)
1857
ORF Size (bp)
1857 bp
RefSeq ID
NM_010583
RefSeq Synonyms
NM_010583, NM_001281968, NM_001281967, NM_001281966, NM_001281965, BC128375, BC128374,
Species
mouse
UniGene ID
Mm.339927
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About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.