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mouse ICT1 shRNA silencing AAV

Name 
AAV-m-ICT1-shRNA
Cat No 
shAAV-261834
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Mrpl58.

shAAV-261834
AAV-m-ICT1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Mrpl58 (mitochondrial ribosomal protein L58). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
93
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
1110001A02Rik; 1110002E03Rik; Ict1; MRP-L58
Description (Vector)
Essential peptidyl-tRNA hydrolase component of the mitochondrial large ribosomal subunit. Acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. May be involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated and thus in the recycling of stalled mitochondrial ribosomes.
Gene ID
68572
Gene Name (Vector)
mitochondrial ribosomal protein L58
Gene Symbol
Mrpl58
ORF Size (aa)
621
ORF Size (bp)
621 bp
RefSeq ID
NM_026729
RefSeq Synonyms
NM_026729, NM_001347645, BC028523,
Species
mouse
UniGene ID
Mm.271062

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.