mouse HIF3A shRNA silencing AAV

Name
AAV-m-HIF3A-shRNA

Cat No
shAAV-261291

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Mouse Hif3a.

shAAV-261291

AAV-m-HIF3A-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Hif3a (hypoxia inducible factor 3, alpha subunit). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

bHLHe17; Ipas; MOP7; NEPAS

Description (Vector)

Involved in adaptive response to hypoxia. Suppresses hypoxia-inducible expression of HIF1A and EPAS1. Binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. Isoform 4 has a dominant-negative function of inactivating HIF1A-mediated transcription. Isoform 4 attenuates the binding of HIF1A to hypoxia-responsive elements (HRE), thus inhibiting HRE-driven transcription. Hypoxia induces down-regulation of isoform 4, leading to activation of HIF1A in hypoxia. Conversely, upon restoring normoxia, the expression of isoform 4 increases and thereby secure an inhibition of HIF1A activity. Isoform 4 may be a negative regulator of hypoxia-inducible gene expression in the kidney and may be involved in renal tumorigenesis. Functions as an inhibitor of angiogenesis in the cornea (By similarity).

Gene ID

53417

Gene Name (Vector)

hypoxia inducible factor 3, alpha subunit

Gene Symbol

Hif3a

ORF Size (aa)

1989

ORF Size (bp)

1989 bp

RefSeq ID

NM_016868

RefSeq Synonyms

NM_016868, NM_001162950, BC120587,

Species

mouse

UniGene ID

Mm.135110

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.