mouse HIF1A shRNA silencing AAV

Name
AAV-m-HIF1A-shRNA

Cat No
shAAV-261289

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Mouse Hif1a.

shAAV-261289

AAV-m-HIF1A-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Hif1a (hypoxia inducible factor 1, alpha subunit). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

AA959795; bHLHe78; HIF-1-alpha; HIF1-alpha; HIF1alpha; MOP1

Description (Vector)

Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.

Gene ID

15251

Gene Name (Vector)

hypoxia inducible factor 1, alpha subunit

Gene Symbol

Hif1a

ORF Size (aa)

2511

ORF Size (bp)

2511 bp

RefSeq ID

NM_010431

RefSeq Synonyms

NM_010431, NM_001313920, NM_001313919, BC026139,

Species

mouse

UniGene ID

Mm.446610

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.