mouse H3F3A shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse H3f3a.
shAAV-261068
AAV-m-H3F3A-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse H3f3a (H3 histone, family 3A). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
H3.3A
Description (Vector)
Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Gene ID
15078
Gene Name (Vector)
H3 histone, family 3A
Gene Symbol
H3f3a
ORF Size (aa)
411
RefSeq ID
NM_008210
RefSeq Synonyms
NM_008210, BC106177, BC088835, BC083353, BC012687, BC002268,
Species
mouse
UniGene ID
Mm.471842
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.