mouse FZD4 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Fzd4.
shAAV-259749
AAV-m-FZD4-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Fzd4 (frizzled class receptor 4). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
Fz4
Description (Vector)
Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin (CTNNB1) canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin (CTNNB1) and activation of Wnt target genes. Plays a critical role in retinal vascularization by acting as a receptor for Wnt proteins and norrin (NDP). In retina, it can be both activated by Wnt protein-binding, but also by a Wnt-independent signaling via binding of norrin (NDP), promoting in both cases beta-catenin (CTNNB1) accumulation and stimulation of LEF/TCF-mediated transcriptional programs. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
Gene ID
14366
Gene Name (Vector)
frizzled class receptor 4
Gene Symbol
Fzd4
ORF Size (aa)
1614
ORF Size (bp)
1614 bp
RefSeq ID
NM_008055
RefSeq Synonyms
NM_008055, BC015256,
Species
mouse
UniGene ID
Mm.86755
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.