mouse EPHB4 shRNA silencing AAV

Name
AAV-m-EPHB4-shRNA

Cat No
shAAV-258582

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Mouse Ephb4.

shAAV-258582

AAV-m-EPHB4-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Ephb4 (Eph receptor B4). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

AI042935; Htk; MDK2; Myk1; Tyro11

Description (Vector)

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Together with its cognate ligand/functional ligand EFNB2 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation form EFNB2-expressing cells. Plays also a role in postnatal blood vessel remodeling, morphogenesis and permeability and is thus important in the context of tumor angiogenesis.

Gene ID

13846

Gene Name (Vector)

Eph receptor B4

Gene Symbol

Ephb4

ORF Size (aa)

2964

ORF Size (bp)

2964 bp

RefSeq ID

NM_010144

RefSeq Synonyms

NM_010144, NM_001159571, BC016505,

Species

mouse

UniGene ID

Mm.34533

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.