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mouse DIAP3 shRNA silencing AAV

Name 
AAV-m-DIAP3-shRNA
Cat No 
shAAV-257175
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Diaph3.

shAAV-257175
AAV-m-DIAP3-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Diaph3 (diaphanous related formin 3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
4930417P13Rik; Dia2; Diap3; Drf3; mDia2; mKIAA4117; p134MDia2
Description (Vector)
Binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics (By similarity).
Gene ID
56419
Gene Name (Vector)
diaphanous related formin 3
Gene Symbol
Diaph3
ORF Size (aa)
2790
ORF Size (bp)
2790 bp
RefSeq ID
NM_019670
RefSeq Synonyms
NM_019670, BC085191, BC028920,
Species
mouse
UniGene ID
Mm.440585

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.