mouse DAPK1 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Dapk1.
shAAV-256803
AAV-m-DAPK1-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Dapk1 (death associated protein kinase 1). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
D13Ucla1; DAP-Kinase
Description (Vector)
Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. Phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. Phosphorylates RPS6, MYL9 and DAPK3. Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-1303 inducing injurious Ca(2+) influx through NMDA receptor channels, resulting in an irreversible neuronal death|Function: Isoform 2 cannot induce apoptosis but can induce membrane blebbing.
Gene ID
69635
Gene Name (Vector)
death associated protein kinase 1
Gene Symbol
Dapk1
ORF Size (aa)
2718
ORF Size (bp)
2718 bp
RefSeq ID
NM_029653
RefSeq Synonyms
NM_134062, NM_029653, NM_001285917, BC060161, BC057317,
Species
mouse
UniGene ID
Mm.24103
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.