mouse CUL4B shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Cul4b.
shAAV-256373
AAV-m-CUL4B-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Cul4b (cullin 4B). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
2700050M05Rik; AA409770; CUL-4B; mKIAA0695
Description (Vector)
Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation-induced DNA damage. Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication. Required for ubiquitination of cyclin E, and consequently, normal G1 cell cycle progression. Regulates the mammalian target-of-rapamycin (mTOR) pathway involved in control of cell growth, size and metabolism. Specific CUL4B regulation of the mTORC1-mediated pathway is dependent upon 26S proteasome function and requires interaction between CUL4B and MLST8.
Gene ID
72584
Gene Name (Vector)
cullin 4B
Gene Symbol
Cul4b
ORF Size (aa)
1845
ORF Size (bp)
1845 bp
RefSeq ID
NM_028288
RefSeq Synonyms
NM_028288, NM_001110142, BC004026,
Species
mouse
UniGene ID
Mm.327675
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.