mouse CUL2 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Cul2.
shAAV-256368
AAV-m-CUL2-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Cul2 (cullin 2). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
1300003D18Rik; 4932411N15Rik; AI327301; mKIAA4106
Description (Vector)
Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).
Gene ID
71745
Gene Name (Vector)
cullin 2
Gene Symbol
Cul2
ORF Size (aa)
2238
ORF Size (bp)
2238 bp
RefSeq ID
NM_029402
RefSeq Synonyms
NM_029402, BC027428, BC026779,
Species
mouse
UniGene ID
Mm.443148
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.