mouse COBRA1 shRNA silencing AAV

Name 
AAV-m-COBRA1-shRNA
Cat No 
shAAV-255821
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Nelfb.

shAAV-255821
AAV-m-COBRA1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Nelfb (negative elongation factor complex member B). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
A730008L03Rik; AB041607; AI663983; Cobra1; Nelf-b
Description (Vector)
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. May be able to induce chromatin unfolding.
Gene ID
58202
Gene Name (Vector)
negative elongation factor complex member B
Gene Symbol
Nelfb
ORF Size (aa)
1428
ORF Size (bp)
1428 bp
RefSeq ID
NM_021393
RefSeq Synonyms
NM_021393, NM_001310157, BC004762,
Species
mouse
UniGene ID
Mm.116418

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About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.