mouse CHKB shRNA silencing AAV

Name 
AAV-m-CHKB-shRNA
Cat No 
shAAV-255404
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Chkb.

shAAV-255404
AAV-m-CHKB-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Chkb (choline kinase beta). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
Chetk; Chkl; Ck; Ck/Ek; Ck/Ek-beta; Ckb; Ckekb; Ek
Description (Vector)
Has a key role in phospholipid biosynthesis. Catalyzes the first step in phosphatidylethanolamine biosynthesis. Phosphorylates ethanolamine, and can also act on choline (in vitro). Has higher activity with ethanolamine. May not significantly contribute to in vivo phosphatidylcholine biosynthesis.
Gene ID
12651
Gene Name (Vector)
choline kinase beta
Gene Symbol
Chkb
ORF Size (aa)
675
ORF Size (bp)
675 bp
RefSeq ID
NM_007692
RefSeq Synonyms
NM_007692, BC068195,
Species
mouse
UniGene ID
Mm.227738

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.