mouse BCKDHB shRNA silencing AAV

Name 
AAV-m-BCKDHB-shRNA
Cat No 
shAAV-253871
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Bckdhb.

shAAV-253871
AAV-m-BCKDHB-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Bckdhb (branched chain ketoacid dehydrogenase E1, beta polypeptide). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
BCKDE1B; BCKDH E1-beta
Description (Vector)
The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).
Gene ID
12040
Gene Name (Vector)
branched chain ketoacid dehydrogenase E1, beta polypeptide
Gene Symbol
Bckdhb
ORF Size (aa)
969
RefSeq ID
NM_199195
RefSeq Synonyms
NM_199195, NM_001305935, BC064099,
Species
mouse
UniGene ID
Mm.12819

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.