mouse AI413782 shRNA silencing AAV

Name 
AAV-m-AI413782-shRNA
Cat No 
shAAV-252308
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Vipas39.

shAAV-252308
AAV-m-AI413782-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Vipas39 (VPS33B interacting protein, apical-basolateral polarity regulator, spe-39 homolog). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
6720456H09Rik; 9330175H22Rik; AI413782; hSPE-39; SPE-39; Spe39; Vipar
Description (Vector)
VIPAS39 may play a role in vesicular trafficking during spermatogenesis (By similarity). Plays a role in lysosomal trafficking, probably via association with the core HOPS complex in a discrete population of endosomes. May play a role in epithelial polarization through stabilization of apical membrane protein content, possibly via the RAB11A-dependent apical recycling pathway. Also involved in direct or indirect transcriptional regulation of E-cadherin.
Gene ID
104799
Gene Name (Vector)
VPS33B interacting protein, apical-basolateral polarity regulator, spe-39 homolog
Gene Symbol
Vipas39
ORF Size (aa)
1419
ORF Size (bp)
1419 bp
RefSeq ID
NM_134044
RefSeq Synonyms
NM_134044, NM_001142581, NM_001142580, BC023716, BC016646,
Species
mouse
UniGene ID
Mm.208750

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.