mouse PHACTR4 shRNA silencing AAV

Name 
AAV-m-PHACTR4-shRNA
Cat No 
shAAV-252290
Availability
6-8 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Phactr4.

shAAV-252290
AAV-m-PHACTR4-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Phactr4 (phosphatase and actin regulator 4). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
3110001B12Rik; AI527228; AW495572; C330013F19Rik; mKIAA4120; N28169
Description (Vector)
Regulator of protein phosphatase 1 (PP1) required for neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development. Acts as an activator of PP1 by interacting with PPP1CA and preventing phosphorylation of PPP1CA at 'Thr-320'. During neural tube closure, localizes to the ventral neural tube and activates PP1, leading to down-regulate cell proliferation within cranial neural tissue and the neural retina. Also acts as a regulator of migration of enteric neural crest cells (ENCCs) by activating PP1, leading to dephosphorylation and subsequent activation of cofilin (COF1 or COF2) and repression of the integrin signaling through the RHO/ROCK pathway (By similarity).
Gene ID
100169
Gene Name (Vector)
phosphatase and actin regulator 4
Gene Symbol
Phactr4
ORF Size (aa)
2085
ORF Size (bp)
2085 bp
RefSeq ID
NM_175306
RefSeq Synonyms
NM_175306, NM_001161797, BC096033, BC075672,
Species
mouse
UniGene ID
Mm.158361

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.