mouse UBE3A shRNA silencing AAV

Name 
AAV-m-UBE3A-shRNA
Cat No 
shAAV-251627
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Ube3a.

shAAV-251627
AAV-m-UBE3A-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Ube3a (ubiquitin protein ligase E3A). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
60
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
4732496B02; 5830462N02Rik; A130086L21Rik; Hpve6a; mKIAA4216
Description (Vector)
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo.
Gene ID
22215
Gene Name (Vector)
ubiquitin protein ligase E3A
Gene Symbol
Ube3a
ORF Size (aa)
2289
ORF Size (bp)
2289 bp
RefSeq ID
NM_011668
RefSeq Synonyms
NM_173010, NM_011668, NM_001033962, BC131659, BC131658,
Species
mouse
UniGene ID
Mm.9002

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.