human BCAT1 shRNA silencing AAV

Name
AAV-h-BCAT1-shRNA

Cat No
shAAV-233187

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human BCAT1.

shAAV-233187

AAV-h-BCAT1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human BCAT1 (branched chain amino acid transaminase 1). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

BCATC; BCT1; ECA39; MECA39; PNAS121; PP18

Description (Vector)

BCAT1 encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described.

Gene ID

586

Gene Name (Vector)

branched chain amino acid transaminase 1

Gene Symbol

BCAT1

HGNC ID

HGNC:976

ORF Size (aa)

1161

ORF Size (bp)

1161 bp

RefSeq ID

NM_005504

RefSeq Synonyms

NM_005504, NM_001178094, NM_001178093, NM_001178092, NM_001178091, BC033864,

Species

human

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.