human MKRN1 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human MKRN1.
shAAV-230144
AAV-h-MKRN1-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human MKRN1 (makorin ring finger protein 1). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
E3 ubiquitin-protein ligase makorin-1; RING finger protein 61; RING-type E3 ubiquitin transferase makorin-1; RNF61
Description (eCommerce)
MKRN1 was identified as a repressor of c-Jun, androgen receptor, and retinoic acid receptor transcriptional activity. MKRN1 is a RING zinc finger protein that has been demonstrated to function as an E3 ubiquitin ligase. The repressive activity of MKRN1 is not dependent on its ubiquitin ligase activity. MKRN1 also appears to function as a transactivator of RNA polymerase II dependent transcription.
Description (Vector)
The Makorin ring finger protein-1 gene (MKRN1) is a highly transcribed, intron-containing source for a family of intronless mammalian genes encoding a novel class of zinc finger proteins. Phylogenetic analyses indicate that the MKRN1 gene is the ancestral founder of this gene family (Gray et al., 2000 (PubMed 10843807)).(supplied by OMIM, Mar 2008)
Gene ID
23608
Gene Name (eCommerce)
makorin ring finger protein 1
Gene Name (Vector)
makorin ring finger protein 1
Gene Symbol
MKRN1
HGNC ID
HGNC:7112
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1449
ORF Size (bp)
1449 bp
Protein Name (eCommerce)
E3 ubiquitin-protein ligase makorin-1
RefSeq ID
NM_013446
RefSeq Synonyms
NM_013446, NM_001291663, NM_001145125, BC064838, BC037400, BC025955,
Research Areas
Antigen Processing and Presentation,Chromatin Remodeling,Immunology,Ubiquitination,Zinc-finger
Research Areas (Faceted)
cell_biology,genetics,immunology
Species
human
Target Sentence
MKRN1 was identified as a repressor of c-Jun, androgen receptor, and retinoic acid receptor transcriptional activity. MKRN1 is a RING zinc finger protein that has been demonstrated to function as an E3 ubiquitin ligase. The repressive activity of MKRN1 is not dependent on its ubiquitin ligase activity. MKRN1 also appears to function as a transactivator of RNA polymerase II dependent transcription.
UniGene ID
Hs.490347
UniProt ID (eCommerce)
Q9UHC7
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.