human ZP3 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human ZP3.
shAAV-228975
AAV-h-ZP3-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human ZP3 (zona pellucida glycoprotein 3). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
Zp-3; ZP3A; ZP3B; ZPC
Description (Vector)
The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a C-terminal consensus furin cleavage site, and a transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus; however, sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3, including the above described variation, and maps closely to this gene.
Gene ID
7784
Gene Name (Vector)
zona pellucida glycoprotein 3
Gene Symbol
ZP3
HGNC ID
HGNC:13189
ORF Size (aa)
1122
RefSeq ID
NM_007155
RefSeq Synonyms
NM_007155, NM_001110354, BC146482, BC140332, BC113949,
Species
human
UniGene ID
Hs.656137
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About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.