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human VPS54 shRNA silencing AAV

Name 
AAV-h-VPS54-shRNA
Cat No 
shAAV-227550
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human VPS54.

shAAV-227550
AAV-h-VPS54-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human VPS54 (VPS54, GARP complex subunit). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
HCC8; hVps54L; PPP1R164; SLP-8p; VPS54L; WR
Description (Vector)
VPS54 encodes for a protein that in yeast forms part of a trimeric vacuolar-protein-sorting complex that is required for retrograde transport of proteins from prevacuoles to the late Golgi compartment. As in yeast, mammalian Vps54 proteins contain a coiled-coil region and dileucine motifs. Alternative splicing results in multiple transcript variants encoding different isoforms
Gene ID
51542
Gene Name (Vector)
VPS54, GARP complex subunit
Gene Symbol
VPS54
HGNC ID
HGNC:18652
ORF Size (aa)
2475
ORF Size (bp)
2475 bp
RefSeq ID
NM_001005739
RefSeq Synonyms
NM_016516, NM_001005739, BC041868, BC030275,
Species
human
UniGene ID
Hs.48499

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About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.