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human VCL shRNA silencing AAV

Name 
AAV-h-VCL-shRNA
Cat No 
shAAV-227426
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human VCL.

shAAV-227426
AAV-h-VCL-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human VCL (vinculin). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
CMD1W; CMH15; epididymis luminal protein 114; epididymis secretory sperm binding protein; HEL114; metavinculin; meta-vinculin; MV; MVCL; vinculin
Description (eCommerce)
Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in vinculin are the cause of cardiomyopathy dilated type 1W, a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia [taken from NCBI Entrez Gene (GeneID: 7414)].
Description (Vector)
Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined.
Gene ID
7414
Gene Name (eCommerce)
vinculin
Gene Name (Vector)
vinculin
Gene Symbol
VCL
HGNC ID
HGNC:12665
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3201
ORF Size (bp)
3201 bp
Protein Name (eCommerce)
Vinculin
RefSeq ID
NM_003373
RefSeq Synonyms
NM_014000, NM_003373, BC039174,
Research Areas
Angiogenesis,Cardiology,Cardiomyopathy,Cardiovascular,Cytoskeleton,EGFR Signaling,Immunology,Rho Signaling,Signal Transduction,VEGF Signaling
Research Areas (Faceted)
cancer,cardiovascular,cell_biology,developmental_biology,immunology,signal_transduction
Species
human
Target Sentence
Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in vinculin are the cause of cardiomyopathy dilated type 1W, a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia [taken from NCBI Entrez Gene (GeneID: 7414)].
UniGene ID
Hs.643896
UniProt ID (eCommerce)
P18206

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.