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human VCAN shRNA silencing AAV

Name 
AAV-h-VCAN-shRNA
Cat No 
shAAV-227425
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human VCAN.

shAAV-227425
AAV-h-VCAN-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human VCAN (versican). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
CSPG2; ERVR; GHAP; PG-M; WGN; WGN1
Description (Vector)
VCAN is a member of the aggrecan/versican proteoglycan family. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and plays a central role in tissue morphogenesis and maintenance. Mutations in this gene are the cause of Wagner syndrome type 1. Multiple transcript variants encoding different isoforms have been found for this gene.
Gene ID
1462
Gene Name (Vector)
versican
Gene Symbol
VCAN
HGNC ID
HGNC:2464
ORF Size (aa)
10188
ORF Size (bp)
10188 bp
RefSeq ID
NM_004385
RefSeq Synonyms
NM_004385, NM_001164098, NM_001164097, NM_001126336, BC172276, BC050524,
Species
human
UniGene ID
Hs.715773

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About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.