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human USP5 shRNA silencing AAV

Name 
AAV-h-USP5-shRNA
Cat No 
shAAV-227340
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human USP5.

shAAV-227340
AAV-h-USP5-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human USP5 (ubiquitin specific peptidase 5). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
deubiquitinating enzyme 5; isopeptidase T; ISOT; testicular tissue protein Li 218; ubiquitin carboxyl-terminal hydrolase 5; ubiquitin isopeptidase T; ubiquitin specific protease 5 (isopeptidase T); ubiquitin thioesterase 5; ubiquitin thiolesterase 5; ubiquitin-specific protease-5 (ubiquitin isopeptidase T); ubiquitin-specific-processing protease 5
Description (eCommerce)
Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 5 (USP5) is a member of this family. The upregulation of USP5 has been found to be associated with fetal Down syndrome.
Description (Vector)
Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 (PubMed 7578059)).(supplied by OMIM, Mar 2010)
Gene ID
8078
Gene Name (eCommerce)
ubiquitin specific peptidase 5
Gene Name (Vector)
ubiquitin specific peptidase 5
Gene Symbol
USP5
HGNC ID
HGNC:12628
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2508
ORF Size (bp)
2508 bp
Protein Name (eCommerce)
Ubiquitin carboxyl-terminal hydrolase 5
RefSeq ID
NM_003481
RefSeq Synonyms
NM_003481, NM_001098536, BC005139, BC004889,
Research Areas
Lysosome,Ubiquitination,Zinc-finger
Research Areas (Faceted)
cell_biology
Species
human
Target Sentence
Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 5 (USP5) is a member of this family. The upregulation of USP5 has been found to be associated with fetal Down syndrome.
UniGene ID
Hs.631661
UniProt ID (eCommerce)
P45974

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.