human USP16 shRNA silencing AAV

Name 
AAV-h-USP16-shRNA
Cat No 
shAAV-227288
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human USP16.

shAAV-227288
AAV-h-USP16-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human USP16 (ubiquitin specific peptidase 16). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
94
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
deubiquitinating enzyme 16; ubiquitin carboxyl-terminal hydrolase 16; ubiquitin specific protease 16; ubiquitin thioesterase 16; ubiquitin thiolesterase 16; ubiquitin-processing protease UBP-M; ubiquitin-specific processing protease 16; Ubiquitin-specific-processing protease 16; UBPM; UBP-M
Description (eCommerce)
Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 16 (USP16) is a member of this family. USP16 activity is cell cycle regulated via phosphorylation and participates in cell cycle control by influencing mitotic chromosome condensation and histone 2A (H2A) deubiquitination.
Description (Vector)
USP16 encodes a deubiquitinating enzyme that is phosphorylated at the onset of mitosis and then dephosphorylated at the metaphase/anaphase transition. It can deubiquitinate H2A, one of two major ubiquitinated proteins of chromatin, in vitro and a mutant form of the protein was shown to block cell division. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Gene ID
10600
Gene Name (eCommerce)
ubiquitin specific peptidase 16
Gene Name (Vector)
ubiquitin specific peptidase 16
Gene Symbol
USP16
HGNC ID
HGNC:12614
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2469
ORF Size (bp)
2469 bp
Protein Name (eCommerce)
Ubiquitin carboxyl-terminal hydrolase 16
RefSeq ID
NM_006447
RefSeq Synonyms
NM_006447, NM_001032410, NM_001001992, BC030777,
Research Areas
Breast Cancer,Cancer,Cell Cycle,Cell Division,DNA Damage/Repair,Histone Modification,Transcription Factor/Regulator,Translational Control,Ubiquitination,Zinc-finger
Research Areas (Faceted)
cancer,cell_biology,cell_cycle,genetics,transcription_translation
Species
human
Target Sentence
Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 16 (USP16) is a member of this family. USP16 activity is cell cycle regulated via phosphorylation and participates in cell cycle control by influencing mitotic chromosome condensation and histone 2A (H2A) deubiquitination.
UniGene ID
Hs.99819
UniProt ID (eCommerce)
Q9Y5T5

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.