human USP1 shRNA silencing AAV

Name 
AAV-h-USP1-shRNA
Cat No 
shAAV-227281
Availability
6-8 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human USP1.

shAAV-227281
AAV-h-USP1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human USP1 (ubiquitin specific peptidase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
deubiquitinating enzyme 1; hUBP; ubiquitin carboxyl-terminal hydrolase 1; ubiquitin specific processing protease 1; ubiquitin specific protease 1; ubiquitin thioesterase 1; ubiquitin thiolesterase 1; Ubiquitin-specific-processing protease 1; UBP
Description (eCommerce)
Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 1 (USP1) has been found to play a role in the Fanconi anemia DNA repair pathway. USP1 specifically removes monoubiquitination from the Fanconi anemia D2 (FANCD2) protein and from the proliferating cell nuclear antigen (PCNA). Deubiquitination of FANCD2 and PCNA is critical to their role in the DNA damage response and DNA crosslink repair.
Description (Vector)
USP1 encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. The protein specifically deubiquitinates a protein in the Fanconi anemia (FA) DNA repair pathway. Alternate transcriptional splice variants have been characterized.
Gene ID
7398
Gene Name (eCommerce)
ubiquitin specific peptidase 1
Gene Name (Vector)
ubiquitin specific peptidase 1
Gene Symbol
USP1
HGNC ID
HGNC:12607
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
2358
ORF Size (bp)
2358 bp
Protein Name (eCommerce)
Ubiquitin carboxyl-terminal hydrolase 1
RefSeq ID
NM_003368
RefSeq Synonyms
NM_003368, NM_001017416, NM_001017415, BC050525,
Research Areas
DNA Damage/Repair,Fanconi anemia,Immunology,Ubiquitination
Research Areas (Faceted)
transcription_translation,cell_biology,immunology,genetics
Species
human
Target Sentence
Ubiquitin (Ub) is a highly conserved protein found ubiquitously in eukaryotic organisms. The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Removal of Ub can rescue proteins from degradation. This is accomplished by the ubiquitin-specific processing protease (UBP) family of enzymes. Ubiquitin specific processing protease 1 (USP1) has been found to play a role in the Fanconi anemia DNA repair pathway. USP1 specifically removes monoubiquitination from the Fanconi anemia D2 (FANCD2) protein and from the proliferating cell nuclear antigen (PCNA). Deubiquitination of FANCD2 and PCNA is critical to their role in the DNA damage response and DNA crosslink repair.
UniGene ID
Hs.35086
UniProt ID (eCommerce)
O94782

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.