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human UCHL3 shRNA silencing AAV

Name 
AAV-h-UCHL3-shRNA
Cat No 
shAAV-227102
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human UCHL3.

shAAV-227102
AAV-h-UCHL3-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human UCHL3 (ubiquitin C-terminal hydrolase L3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
testicular tissue protein Li 221; ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase); ubiquitin carboxyl-terminal hydrolase isozyme L3; ubiquitin thioesterase L3; ubiquitin thiolesterase; UCH-L3
Description (eCommerce)
Ubiquitin carboxyl-terminal hydrolase isoenzyme 3 (UCH-L3) is a deubiquitinating enzyme (DUB) and thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCH-L3 deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ P15374].
Description (Vector)
UCHL3 is a member of the deubiquitinating enzyme family. Members of this family are proteases that catalyze the removal of ubiquitin from polypeptides and are divided into five classes, depending on the mechanism of catalysis. This protein may hydrolyze the ubiquitinyl-N-epsilon amide bond of ubiquitinated proteins to regenerate ubiquitin for another catalytic cycle. Alternative splicing results in multiple transcript variants that encode different protein isoforms.
Gene ID
7347
Gene Name (eCommerce)
ubiquitin C-terminal hydrolase L3
Gene Name (Vector)
ubiquitin C-terminal hydrolase L3
Gene Symbol
UCHL3
HGNC ID
HGNC:12515
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
693
Protein Name (eCommerce)
Ubiquitin carboxyl-terminal hydrolase isozyme L3
RefSeq ID
NM_006002
RefSeq Synonyms
NM_006002, NM_001270952, BC018125,
Research Areas
Complexome,Mitochondrion,Proteasome,Ubiquitination
Research Areas (Faceted)
cell_biology
Species
human
Target Sentence
Ubiquitin carboxyl-terminal hydrolase isoenzyme 3 (UCH-L3) is a deubiquitinating enzyme (DUB) and thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCH-L3 deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ P15374].
UniGene ID
Hs.162241
UniProt ID (eCommerce)
P15374

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.