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human UBE2H shRNA silencing AAV

Name 
AAV-h-UBE2H-shRNA
Cat No 
shAAV-227012
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human UBE2H.

shAAV-227012
AAV-h-UBE2H-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human UBE2H (ubiquitin conjugating enzyme E2 H). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
E2-20K; GID3; UBC8; UBCH; UBCH2
Description (Vector)
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Three alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.
Gene ID
7328
Gene Name (Vector)
ubiquitin conjugating enzyme E2 H
Gene Symbol
UBE2H
HGNC ID
HGNC:12484
ORF Size (aa)
552
RefSeq ID
NM_003344
RefSeq Synonyms
NM_182697, NM_003344, NM_001202498, BC006277,
Species
human
UniGene ID
Hs.643548

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.