human TDG shRNA silencing AAV

Name 
AAV-h-TDG-shRNA
Cat No 
shAAV-225260
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human TDG.

shAAV-225260
AAV-h-TDG-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human TDG (thymine DNA glycosylase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
G/T mismatch-specific thymine DNA glycosylase; hTDG; Thymine-DNA glycosylase
Description (eCommerce)
Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine [taken from NCBI Entrez Gene (Gene ID: 6996)].
Description (Vector)
TDG belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12.
Gene ID
6996
Gene Name (eCommerce)
thymine DNA glycosylase
Gene Name (Vector)
thymine DNA glycosylase
Gene Symbol
TDG
HGNC ID
HGNC:11700
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1233
ORF Size (bp)
1233 bp
Protein Name (eCommerce)
G/T mismatch-specific thymine DNA glycosylase
RefSeq ID
NM_003211
RefSeq Synonyms
NM_003211, BC037557,
Research Areas
Chromatin Remodeling,DNA Damage/Repair,Epigenetic Regulation of Gene Expression,Immunology,Transcription Factor/Regulator
Research Areas (Faceted)
cell_biology,immunology,genetics,transcription_translation
Species
human
Target Sentence
Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine [taken from NCBI Entrez Gene (Gene ID: 6996)].
UniGene ID
Hs.584809
UniProt ID (eCommerce)
Q13569

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.