human TAF12 shRNA silencing AAV

Name
AAV-h-TAF12-shRNA

Cat No
shAAV-224912

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human TAF12.

shAAV-224912

AAV-h-TAF12-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human TAF12 (TATA-box binding protein associated factor 12). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

TAF2J; TAFII20

Description (Vector)

Control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins. These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site. Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors. Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex. Among the latter are the TAF proteins. Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I. Two transcript variants encoding the same protein have been found for this gene.

Gene ID

6883

Gene Name (Vector)

TATA-box binding protein associated factor 12

Gene Symbol

TAF12

HGNC ID

HGNC:11545

ORF Size (aa)

486

ORF Size (bp)

486 bp

RefSeq ID

NM_005644

RefSeq Synonyms

NM_005644, NM_001135218, BC011986,

Species

human

UniGene ID

Hs.530251

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.