human SVIL shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human SVIL.
shAAV-224784
AAV-h-SVIL-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human SVIL (supervillin). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Description (Vector)
SVIL encodes a bipartite protein with distinct amino- and carboxy-terminal domains. The amino-terminus contains nuclear localization signals and the carboxy-terminus contains numerous consecutive sequences with extensive similarity to proteins in the gelsolin family of actin-binding proteins, which cap, nucleate, and/or sever actin filaments. The gene product is tightly associated with both actin filaments and plasma membranes, suggesting a role as a high-affinity link between the actin cytoskeleton and the membrane. The encoded protein appears to aid in both myosin II assembly during cell spreading and disassembly of focal adhesions. Two transcript variants encoding different isoforms of supervillin have been described.
Gene ID
6840
Gene Name (Vector)
supervillin
Gene Symbol
SVIL
HGNC ID
HGNC:11480
ORF Size (aa)
6645
ORF Size (bp)
6645 bp
RefSeq ID
NM_003174
RefSeq Synonyms
NM_021738, NM_003174, NM_001323600, NM_001323599, BC172362,
Species
human
UniGene ID
Hs.499209
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.