human SURF1 shRNA silencing AAV

Name
AAV-h-SURF1-shRNA

Cat No
shAAV-224757

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human SURF1.

shAAV-224757

AAV-h-SURF1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human SURF1 (SURF1, cytochrome c oxidase assembly factor). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

CMT4K

Description (Vector)

SURF1 encodes a protein localized to the inner mitochondrial membrane and thought to be involved in the biogenesis of the cytochrome c oxidase complex. The protein is a member of the SURF1 family, which includes the related yeast protein SHY1 and rickettsial protein RP733. The gene is located in the surfeit gene cluster, a group of very tightly linked genes that do not share sequence similarity, where it shares a bidirectional promoter with SURF2 on the opposite strand. Defects in this gene are a cause of Leigh syndrome, a severe neurological disorder that is commonly associated with systemic cytochrome c oxidase deficiency.

Gene ID

6834

Gene Name (Vector)

SURF1, cytochrome c oxidase assembly factor

Gene Symbol

SURF1

HGNC ID

HGNC:11474

ORF Size (aa)

903

RefSeq ID

NM_003172

RefSeq Synonyms

NM_003172, NM_001280787, BC071658, BC028314,

Species

human

UniGene ID

Hs.512464

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.