human ST8SIA4 shRNA silencing AAV

Name 
AAV-h-ST8SIA4-shRNA
Cat No 
shAAV-224486
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human ST8SIA4.

shAAV-224486
AAV-h-ST8SIA4-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ST8SIA4 (ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
PST; PST1; SIAT8D; ST8SIA-IV
Description (Vector)
ST8SIA4 catalyzes the polycondensation of alpha-2,8-linked sialic acid required for the synthesis of polysialic acid, a modulator of the adhesive properties of neural cell adhesion molecule (NCAM1). The encoded protein, which is a member of glycosyltransferase family 29, is a type II membrane protein that may be present in the Golgi apparatus. Two transcript variants encoding different isoforms have been found for this gene.
Gene ID
7903
Gene Name (Vector)
ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4
Gene Symbol
ST8SIA4
HGNC ID
HGNC:10871
ORF Size (aa)
1080
RefSeq ID
NM_005668
RefSeq Synonyms
NM_175052, NM_005668, BC053657, BC027866,
Species
human
UniGene ID
Hs.308628

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.