human SPAM1 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human SPAM1.
shAAV-224074
AAV-h-SPAM1-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human SPAM1 (sperm adhesion molecule 1). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
HEL-S-96n; HYA1; HYAL1; HYAL3; HYAL5; PH-20; PH20; SPAG15
Description (Vector)
Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene.
Gene ID
6677
Gene Name (Vector)
sperm adhesion molecule 1
Gene Symbol
SPAM1
HGNC ID
HGNC:11217
ORF Size (aa)
1536
ORF Size (bp)
1536 bp
RefSeq ID
NM_003117
RefSeq Synonyms
NM_153189, NM_003117, NM_001174046, NM_001174045, NM_001174044, BC026163,
Species
human
UniGene ID
Hs.121494
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About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.