human SIRT1 shRNA silencing AAV

Name 
AAV-h-SIRT1-shRNA
Cat No 
shAAV-223014
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human SIRT1.

shAAV-223014
AAV-h-SIRT1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human SIRT1 (sirtuin 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
hSIR2; hSIRT1; NAD-dependent protein deacetylase sirtuin-1; regulatory protein SIR2 homolog 1; SIR2; SIR2alpha; SIR2L1; SIR2-like protein 1; sirtuin type 1
Description (eCommerce)
Sirt1 is one of seven members of the sirtuin family of proteins that are homologous to the yeast silent information regulator 2 (Sir2), a NAD+-dependent protein deacetylase that plays a role in lifespan in lower eukaryotes. The sirtuins are diverse in their cellular functions, and are important to the regulation of oxidative stress, and metabolism. Sirt 1 is localized to the nucleus and possesses deacetylase activity. Sirt 1 has been shown to be able to deacetylate p53, Ku70, NF-kappaB and forkhead proteins to mediate cellular resistance to stress.
Gene ID
23411
Gene Name (eCommerce)
sirtuin 1
Gene Name (Vector)
sirtuin 1
Gene Symbol
SIRT1
HGNC ID
HGNC:14929
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1668
ORF Size (bp)
1668 bp
Protein Name (eCommerce)
NAD-dependent protein deacetylase sirtuin-1
RefSeq ID
NM_012238
RefSeq Synonyms
NM_012238, NM_001314049, NM_001142498, BC012499,
Research Areas
Aging,Angiogenesis,Apoptosis,Autophagy,Breast Cancer,Cancer,Cell Cycle,Chromatin Remodeling,DNA Damage/Repair,DNA Replication,Epigenetic Regulation of Gene Expression,FoxO Signaling,Helicase Activity,Histone Modification,Host-Virus Interactions,Hypoxia,Immunology,Insulin Signaling,Metabolism/Metabolic Process,MicroRNAs in Cancer,Mitochondrion,NF-kB Signaling,RNA Processing,Signal Transduction,Spermatogenesis,Transcription Factor/Regulator
Research Areas (Faceted)
cancer,cell_biology,cell_cycle,developmental_biology,genetics,transcription_translation,immunology,infectious_disease,metabolism,signal_transduction
Species
human
Target Sentence
Sirt1 is one of seven members of the sirtuin family of proteins that are homologous to the yeast silent information regulator 2 (Sir2), a NAD+-dependent protein deacetylase that plays a role in lifespan in lower eukaryotes. The sirtuins are diverse in their cellular functions, and are important to the regulation of oxidative stress, and metabolism. Sirt 1 is localized to the nucleus and possesses deacetylase activity. Sirt 1 has been shown to be able to deacetylate p53, Ku70, NF-kappaB and forkhead proteins to mediate cellular resistance to stress.
UniGene ID
Hs.369779
UniProt ID (eCommerce)
Q96EB6

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.