human SEPHS2 shRNA silencing AAV

Name
AAV-h-SEPHS2-shRNA

Cat No
shAAV-222585

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human SEPHS2.

shAAV-222585

AAV-h-SEPHS2-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human SEPHS2 (selenophosphate synthetase 2). Available with optional GFP reporter or cell-specific promoter.

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Vector Designer

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

SPS2; SPS2b

Description (Vector)

SEPHS2 encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons. This protein itself contains a selenocysteine residue in its predicted active site. The 3' UTR of the gene has a stem-loop secondary structure called a selenocysteine insertion sequence (SECIS) element, which allows UGA to direct the incorporation of selenocysteine rather than signal a translational stop. Alternatively spliced transcripts have been identified, but their biological validity has not been determined.

Gene ID

22928

Gene Name (Vector)

selenophosphate synthetase 2

Gene Symbol

SEPHS2

HGNC ID

HGNC:19686

ORF Size (aa)

1347

ORF Size (bp)

1347 bp

RefSeq ID

NM_012248

RefSeq Synonyms

NM_012248, BC016643, BC002381,

Species

human

UniGene ID

Hs.718460

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.