human RTN3 shRNA silencing AAV

Name 
AAV-h-RTN3-shRNA
Cat No 
shAAV-222089
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human RTN3.

shAAV-222089
AAV-h-RTN3-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human RTN3 (reticulon 3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
ASY interacting protein; ASYIP; HAP; homolog of ASY protein; neuroendocrine-specific protein-like 2; neuroendocrine-specific protein-like II; NSPL2; NSPLII; NSP-like protein 2; NSP-like protein II; reticulon-3; RTN3-A1
Description (eCommerce)
Reticulon-3 may be involved in membrane trafficking in the early secretory pathway. Reticulon-3 inhibits BACE1 activity and amyloid precursor protein processing. It may induce caspase-8 cascade and apoptosis and may favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress. In case of enteroviruses infection, Reticulon-3 may be involved in the viral replication or pathogenesis [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ O95197].
Description (Vector)
RTN3 belongs to the reticulon family of highly conserved genes that are preferentially expressed in neuroendocrine tissues. This family of proteins interact with, and modulate the activity of beta-amyloid converting enzyme 1 (BACE1), and the production of amyloid-beta. An increase in the expression of any reticulon protein substantially reduces the production of amyloid-beta, suggesting that reticulon proteins are negative modulators of BACE1 in cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene, and pseudogenes of this gene are located on chromosomes 4 and 12.
Gene ID
10313
Gene Name (eCommerce)
reticulon 3
Gene Name (Vector)
reticulon 3
Gene Symbol
RTN3
HGNC ID
HGNC:10469
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3039
ORF Size (bp)
3039 bp
Protein Name (eCommerce)
Reticulon-3
RefSeq ID
NM_006054
RefSeq Synonyms
NM_201430, NM_201429, NM_201428, NM_006054, NM_001265591, NM_001265590, NM_001265589, BC148632, BC010556,
Research Areas
Apoptosis,Golgi Apparatus,Host-Virus Interactions,Neurobiology,Transport
Research Areas (Faceted)
cell_biology,infectious_disease,neurobiology
Species
human
Target Sentence
Reticulon-3 may be involved in membrane trafficking in the early secretory pathway. Reticulon-3 inhibits BACE1 activity and amyloid precursor protein processing. It may induce caspase-8 cascade and apoptosis and may favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress. In case of enteroviruses infection, Reticulon-3 may be involved in the viral replication or pathogenesis [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ O95197].
UniGene ID
Hs.473761
UniProt ID (eCommerce)
O95197

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.