human RPS14 shRNA silencing AAV

Name 
AAV-h-RPS14-shRNA
Cat No 
shAAV-221827
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human RPS14.

shAAV-221827
AAV-h-RPS14-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human RPS14 (ribosomal protein S14). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
40S ribosomal protein S14; emetine resistance; EMTB; S14; small ribosomal subunit protein uS11
Description (eCommerce)
Ribosomal protein S14 (RPS14) belongs to the S11P family of ribosomal proteins. It is located in the cytoplasm [taken from NCBI Entrez Gene (Gene ID: 6208)].
Description (Vector)
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S11P family of ribosomal proteins. It is located in the cytoplasm. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. In Chinese hamster ovary cells, mutations in this gene can lead to resistance to emetine, a protein synthesis inhibitor. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
Gene ID
6208
Gene Name (eCommerce)
ribosomal protein S14
Gene Name (Vector)
ribosomal protein S14
Gene Symbol
RPS14
HGNC ID
HGNC:10387
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
456
Protein Name (eCommerce)
40S ribosomal protein S14
RefSeq ID
NM_005617
RefSeq Synonyms
NM_005617, NM_001025071, NM_001025070, BC091474, BC020515, BC006784, BC003401, BC001126,
Research Areas
Gene Expression,Host-Virus Interactions,Metabolism/Metabolic Process,Mitochondrion,mRNA Binding,RNA Binding,RNA Processing,Translational Control
Research Areas (Faceted)
genetics,transcription_translation,infectious_disease,metabolism,cell_biology,genetics,transcription_translation
Species
human
Target Sentence
Ribosomal protein S14 (RPS14) belongs to the S11P family of ribosomal proteins. It is located in the cytoplasm [taken from NCBI Entrez Gene (Gene ID: 6208)].
UniGene ID
Hs.381126
UniProt ID (eCommerce)
P62263

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.