human RPN1 shRNA silencing AAV

Name
AAV-h-RPN1-shRNA

Cat No
shAAV-221787

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human RPN1.

shAAV-221787

AAV-h-RPN1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human RPN1 (ribophorin I). Available with optional GFP reporter or cell-specific promoter.

Request a Quote

Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

dolichyl-diphosphooligosaccharide-protein glycosyltransferase 67 kDa subunit; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit; dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1; oligosaccharyltransferase 1 homolog; oligosaccharyltransferase complex subunit (non-catalytic); OST1; RBPH1; Ribophorin I; ribophorin-1; RPN-I

Description (eCommerce)

Ribophorin I is an essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains [taken from NCBI Entrez Gene (Gene ID: 6184)].

Description (Vector)

RPN1 encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome.

Gene ID

6184

Gene Name (eCommerce)

ribophorin I

Gene Name (Vector)

ribophorin I

Gene Symbol

RPN1

HGNC ID

HGNC:10381

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

1824

ORF Size (bp)

1824 bp

Protein Name (eCommerce)

Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1

RefSeq ID

NM_002950

RefSeq Synonyms

NM_002950, BC010839,

Research Areas

Complexome,Gene Expression,Metabolism/Metabolic Process,Proteasome,Signal Transduction,Translational Control

Research Areas (Faceted)

cell_biology,metabolism,signal_transduction,transcription_translation

Species

human

Target Sentence

UniGene ID

Hs.603636

UniProt ID (eCommerce)

P04843

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.