human RIPPLY1 shRNA silencing AAV

Name
AAV-h-RIPPLY1-shRNA

Cat No
shAAV-221262

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human RIPPLY1.

shAAV-221262

AAV-h-RIPPLY1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human RIPPLY1 (ripply transcriptional repressor 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Description (Vector)

RIPPLY1 encodes a protein similar to a zebrafish protein which acts as a transcriptional repressor in and is required for somite segmentation in zebrafish embryos (PMID: 16326386). Multiple transcript variants encoding different isoforms have been found for this gene.

Gene ID

92129

Gene Name (Vector)

ripply transcriptional repressor 1

Gene Symbol

RIPPLY1

HGNC ID

HGNC:25117

ORF Size (aa)

438

RefSeq ID

NM_138382

RefSeq Synonyms

NM_138382, NM_001171706, BC127250, BC110436, BC105692, BC105691,

Species

human

UniGene ID

Hs.334726

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.