human RFX5 shRNA silencing AAV

Name 
AAV-h-RFX5-shRNA
Cat No 
shAAV-221073
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human RFX5.

shAAV-221073
AAV-h-RFX5-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human RFX5 (regulatory factor X5). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
DNA-binding protein RFX5; regulatory factor X 5; regulatory factor X, 5 (influences HLA class II expression)
Description (eCommerce)
Regulatory factor X-5 (RFX5) is a 75kDa subunit of the regulatory factor X (RFX) heteromeric complex that activates the transcription of major histocompatibility class II (MHC-II) genes by binding MHC-II promoter elements. The RFX complex consists of RFX5, RFXANK, and RFXAP. Mutations in these genes result in a lack of MHCII transcription and lead to the severe combined immunodeficiency disease, Bare Lymphocyte Syndrome (BLS).
Description (Vector)
A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS; MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX, a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX (Steimle et al., 1995). RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined.
Gene ID
5993
Gene Name (eCommerce)
regulatory factor X5
Gene Name (Vector)
regulatory factor X5
Gene Symbol
RFX5
HGNC ID
HGNC:9986
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1851
ORF Size (bp)
1851 bp
Protein Name (eCommerce)
DNA-binding protein RFX5
RefSeq ID
NM_000449
RefSeq Synonyms
NM_001025603, NM_000449, BC017471,
Research Areas
Antigen Processing and Presentation,Immunology,Nuclear Receptor Coactivators,Transcription Factor/Regulator
Research Areas (Faceted)
cell_biology,immunology,transcription_translation
Species
human
Target Sentence
Regulatory factor X-5 (RFX5) is a 75kDa subunit of the regulatory factor X (RFX) heteromeric complex that activates the transcription of major histocompatibility class II (MHC-II) genes by binding MHC-II promoter elements. The RFX complex consists of RFX5, RFXANK, and RFXAP. Mutations in these genes result in a lack of MHCII transcription and lead to the severe combined immunodeficiency disease, Bare Lymphocyte Syndrome (BLS).
UniGene ID
Hs.632472
UniProt ID (eCommerce)
P48382

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.