human RAG1 shRNA silencing AAV

Name 
AAV-h-RAG1-shRNA
Cat No 
shAAV-220670
Availability
6-8 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human RAG1.

shAAV-220670
AAV-h-RAG1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human RAG1 (recombination activating 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
RAG-1; RNF74
Description (Vector)
RAG1 is involved in activation of immunoglobulin V-D-J recombination. The encoded protein is involved in recognition of the DNA substrate, but stable binding and cleavage activity also requires RAG2. Defects in this gene can be the cause of several diseases.
Gene ID
5896
Gene Name (Vector)
recombination activating 1
Gene Symbol
RAG1
HGNC ID
HGNC:9831
ORF Size (aa)
2796
ORF Size (bp)
2796 bp
RefSeq ID
NM_000448
RefSeq Synonyms
NM_000448, BC037344,
Species
human
UniGene ID
Hs.677010

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.