human PRPF4 shRNA silencing AAV

Name 
AAV-h-PRPF4-shRNA
Cat No 
shAAV-219960
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human PRPF4.

shAAV-219960
AAV-h-PRPF4-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PRPF4 (pre-mRNA processing factor 4). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
50
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
HPRP4; HPRP4P; PRP4; Prp4p; RP70; SNRNP60
Description (Vector)
PRPF4 is part of a heteromeric complex that binds U4, U5, and U6 small nuclear RNAs and is involved in pre-mRNA splicing. The encoded protein also is a mitotic checkpoint protein and a regulator of chemoresistance in human ovarian cancer. Two transcript variants encoding different isoforms have been found for this gene.
Gene ID
9128
Gene Name (Vector)
pre-mRNA processing factor 4
Gene Symbol
PRPF4
HGNC ID
HGNC:17349
ORF Size (aa)
1569
ORF Size (bp)
1569 bp
RefSeq ID
NM_004697
RefSeq Synonyms
NM_004697, NM_001322267, NM_001322266, NM_001244926, BC007424, BC001588,
Species
human
UniGene ID
Hs.530284

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.