human PPEF1 shRNA silencing AAV

Name 
AAV-h-PPEF1-shRNA
Cat No 
shAAV-219522
Availability
6-8 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human PPEF1.

shAAV-219522
AAV-h-PPEF1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human PPEF1 (protein phosphatase with EF-hand domain 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
PP7; PPEF; PPP7C; PPP7CA
Description (Vector)
PPEF1 encodes a member of the serine/threonine protein phosphatase with EF-hand motif family. The protein contains a protein phosphatase catalytic domain, and at least two EF-hand calcium-binding motifs in its C terminus. Although its substrate(s) is unknown, the encoded protein has been suggested to play a role in specific sensory neuron function and/or development. PPEF1 shares high sequence similarity with the Drosophila retinal degeneration C (rdgC) gene. Several alternatively spliced transcript variants, each encoding a distinct isoform, have been described.
Gene ID
5475
Gene Name (Vector)
protein phosphatase with EF-hand domain 1
Gene Symbol
PPEF1
HGNC ID
HGNC:9243
ORF Size (aa)
1962
ORF Size (bp)
1962 bp
RefSeq ID
NM_006240
RefSeq Synonyms
NM_152226, NM_152224, NM_006240, BC036026,
Species
human
UniGene ID
Hs.211589

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.