human POLR3F shRNA silencing AAV

Name
AAV-h-POLR3F-shRNA

Cat No
shAAV-219420

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human POLR3F.

shAAV-219420

AAV-h-POLR3F-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human POLR3F (RNA polymerase III subunit F). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

RPC39; RPC6

Description (Vector)

POLR3F is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase.

Gene ID

10621

Gene Name (Vector)

RNA polymerase III subunit F

Gene Symbol

POLR3F

HGNC ID

HGNC:15763

ORF Size (aa)

951

ORF Size (bp)

951 bp

RefSeq ID

NM_006466

RefSeq Synonyms

NM_006466, NM_001282526, BC016761, BC012588,

Species

human

UniGene ID

Hs.472227

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.