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human POLR2F shRNA silencing AAV

Name 
AAV-h-POLR2F-shRNA
Cat No 
shAAV-219396
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human POLR2F.

shAAV-219396
AAV-h-POLR2F-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human POLR2F (RNA polymerase II subunit F). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
HRBP14.4; POLRF; RPABC14.4; RPABC2; RPB14.4; RPB6; RPC15
Description (Vector)
POLR2F encodes the sixth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes, that is also shared by the other two DNA-directed RNA polymerases. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template.
Gene ID
5435
Gene Name (Vector)
RNA polymerase II subunit F
Gene Symbol
POLR2F
HGNC ID
HGNC:9193
ORF Size (aa)
384
RefSeq ID
NM_021974
RefSeq Synonyms
NM_021974, NM_001301131, NM_001301130, NM_001301129, BC003582,
Species
human
UniGene ID
Hs.436578

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.